期刊
IMMUNOLOGY AND CELL BIOLOGY
卷 97, 期 10, 页码 877-887出版社
WILEY
DOI: 10.1111/imcb.12286
关键词
B cells; CD40 signaling; humoral immune responses; p190RhoGEF; plasma cells
资金
- National Research Foundation of Korea (NRF) - Korean Government [2009-0067361, 2011-0016543, 2015R1A2A2A01004209, 2019R1A2C1007743, 2012R1A5A1048236]
- Brain Korea 21 Program of the Korea Ministry of Education
- National Research Foundation of Korea [2011-0016543, 2009-0067361, 2015R1A2A2A01004209, 2019R1A2C1007743] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Previously, we reported induced expression of the p190 Rho guanine nucleotide exchange factor (p190RhoGEF, ARHGEF28) following CD40 stimulation of B cells isolated from mouse spleen. We also reported that p190RhoGEF and a downstream effector molecule RhoA are required for B-cell differentiation, especially for the induction of the plasma cell (PC) differentiation. This study investigates the role of p190RhoGEF in B-cell biology in vivo, using p190RhoGEF transgenic (TG) mice that overexpress a wild-type full gene in B cells. Immunization of these mice with T-cell-dependent antigen showed that populations of germinal center B cells and PCs were significantly increased in TG mice. Furthermore, similar results were shown in recombination activating 1 (Rag1) knockout mice that were reconstituted with B cells isolated from TG mice in combination with T cells isolated from littermate control mice. Analyses of isotype class switching and transcription factors involved in a germinal center reaction and PC differentiation also supported the findings from the cellular responses. These results suggest that p190RhoGEF may play a role in the stage of PC differentiation during T-cell-dependent humoral immune responses.
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