期刊
IEEE TRANSACTIONS ON NANOBIOSCIENCE
卷 18, 期 3, 页码 369-372出版社
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TNB.2019.2920743
关键词
Fine needle aspiration biopsy; electrical impedance spectroscopy; fine needle aspirate (FNA); single cell analysis; microfluidic flow cytometry; label-free detection; cell enumeration; patient-derived cells
资金
- Draper Laboratory
Fine needle aspirate sampling of tumors requires acquisition of sufficient cells to complete a diagnosis. Aspirates through such fine needles are typically composed of small cell clusters in suspension, making them readily amenable to microfluidic analysis. Here we show a microfluidic device with integrated electrodes capable of interrogating and identifying cellular components in a patient-derived sample of dissociated tumor cells using micro-electrical impedance spectroscopy (mu EIS). We show that the mu EIS system can distinguish dissociated tumor cells in a sample consisting of red blood cell (RBCs) and peripheral blood mononucleated cells (PBMCs). Our mu EIS system can also distinguish dissociated tumor cells from normal cells and we show results for five major cancer types, specifically, lung, thyroid, breast, ovarian, and kidney cancer. Moreover, our mu EIS system can make these distinctions in a label-free manner, thereby opening the possibility of integration into standard clinical workflows at the point of care.
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