期刊
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
卷 66, 期 6, 页码 1742-1751出版社
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TBME.2018.2878887
关键词
Adult zebrafish; high-frequency ultrasound; ultrafast ultrasound imaging; doppler imaging; adaptive spatiotemporal filtering
资金
- National Health Research Institutes in Taiwan [NHRI-EX107-10712EI]
- Ministry of Science and Technology of Taiwan [MOST 107-2221-E-006-024-MY3]
- Medical Device Innovation Center, National Cheng Kung University from the Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education in Taiwan
Objective: Zebrafish has been recently considered an ideal vertebrate for studying developmental biology, genetics, particularly for modeling tumorigenesis, angiogenesis, and regeneration in vivo. However, when a zebrafish matures completely, its body loses transparency, thus making conventional optical imaging techniques difficult for imaging internal anatomy and vasculature. Acoustic wave penetration outperforms optical methods, high-frequency (>30 MHz) ultrasound (HFUS) was consequently an alternative imaging modality for adult zebrafish imaging, particularly for echocardiography However, visualizing peripheral vessels in a zebrafish by using conventional HFUS is still difficult. Methods: In the present study, high-frequency micro-Doppler imaging (HF mu DI) based on ultrafast ultrasound imaging was proposed for zebrafish dorsal vascular mapping in vivo. HF mu DI uses a 40-MHz ultrasound transducer, which is an ultrafast ultrasound imaging technology with the highest frequency available currently. Blood flow signals were extracted using an eigen-based clutter filter with different settings. Experiments were performed on an 8-month-old wild-type AB-line adult zebrafish. Results: Blood vessels, including intersegmental vessels, parachordal vessel, dorsal longitudinal anastomotic vessel, and dorsal aorta, from the dorsal side of the zebrafish were clearly observed in two-dimensional (2-D) and 3-D HF mu DI. Conclusion: The maximum image depth of HF mu DI and the minimal diameter of vessel can be detected were 4 mm and 36 mu m, respectively; they were determined without any use of microbubbles. The maximum flow velocity range was approximately 3-4 mm/s on the dorsal vessels of the adult zebrafish. Significance: Compared with conventional ultrasound Doppler imaging, HF mu DI exhibited superior small vessel imaging.
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