Clumped isotopes in land snail shells over China: Towards establishing a biogenic carbonate paleothermometer

Land snail fossils are abundantly distributed in geological deposits and their isotopic compositions provide a means to determine paleoclimatic changes. With the development of the clumped isotopes (Delta(47)) geothermometer, many efforts have been made in recent years to study clumped isotopes in land snail shell carbonate. Although there have been several recent attempts, there is, as yet, no empirical calibration function to convert land snail Delta(47) to environmental temperature. Here, we systematically analyzed clumped isotopes (Delta(47)) of two common land snail species (Bradybaena and Cathaica) from China. Results showed that temperatures calculated using the Delta(47) (T-47) of both species did not correlate with the mean annual temperatures (MAT) at the study sites. However, the T-47-MAT offset is negatively correlated to MAT, suggesting that land snails tend to add shell during the warmer months at colder sites or modulate their body temperature differently in colder regions. Meanwhile, clumped temperatures of Cathaica are 3.4 +/- 1.5 degrees C higher than those of Bradybaena at 18 sites, indicating that a species-specific transfer function is needed to reconstruct paleotemperature using land snail clumped isotopes. After determining the proper duration of the growing season for land snails at different locations, we developed a Delta(47)-growth season temperature (GST) transfer function for the two species. The calibration function for Bradybaena land snails is expressed by a linear regression between 1/T-2 and absolute Delta(47) (R-2 = 0.94): Delta(47) = (0.0513 +/- 0.0036) x 10(6)/T-2 + (0.0930 +/- 0.0413), where Delta(47) is expressed in %0 and T in K. The calibration function for Cathaica is as follows (R-2 = 0.80): Delta(47) = (0.055 +/- 0.011) x 10(6)/T-2 + (0.035 +/- 0.129). The function for Cathaica was successfully applied to reconstruct mean summer (June-July-August) temperatures during the Last Glacial Maximum and modern times on the central Chinese Loess Plateau, based on Delta(47) data of Cathaica sp. provided by Eagle et al (2013a). This testifies to the validity of the aforementioned constructed transfer function. In addition, the calculated delta O-18 of body water (delta O-18(BW)) for Bradybaena showed a robust correlation with the delta O-18 of rainfall (delta O-18(p)), particularly in northern China, which points to the potential to trace hydrological changes in the region. In contrast, Cathaica delta O-18(BW) did not show a straightforward relation to delta O-18(p). This inter-species complexity warrants further study. (C) 2019 Elsevier Ltd. All rights reserved.