期刊
GENE
卷 706, 期 -, 页码 84-90出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gene.2019.04.067
关键词
Flammulina filiformis; Hydrophobins; Hyd9; Fungal primordia formation; Fruiting body development
资金
- National Basic Research Program of China [2014CB138302]
- Breeding and Industrialization Project of Wood Rot Edible Fungi [fjzycxny2017010]
- Young Academic Backbone Training Fund in the College of Horticulture at Fujian Agriculture and Forestry University [61201400706]
- Science and Technology Innovation Special Fund at Fujian Agriculture and Forestry University [CXZX2017184]
Flammulina filiformis is an edible fungus that is largely cultivated and widely consumed around the world. The quantity and quality of the primordia, which gives rise to the fruiting body, affects its production efficiency. Hydrophobins are involved in the formation of the fruiting body of macrofungi. However, functional verification of the hydrophobin genes is limited to date. In this study, we used gene silencing and overexpression analyses to investigate the function of one F. filiformis hydrophobin gene (Hyd9) during the development of the fruiting body. The Hyd9-silenced transformants exhibited sparse aerial hyphae, resulting in fewer primordia and fruiting bodies. In contrast, the Hyd9 overexpression strain displayed denser aerial hyphae and more primordia. The phenotypes of these transgenic lines strongly suggested that Hyd9 plays an important role in the formation of aerial hyphal knots (the primary stage of primordia) and primordia in F. filiformis. These results will be beneficial for developing more efficient methods to induce primordia formation in F. filiformis and other commercially valuable mushrooms.
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