期刊
FOOD RESEARCH INTERNATIONAL
卷 121, 期 -, 页码 197-204出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.foodres.2019.03.035
关键词
Antarctic krill (Euphausia superba); Peptides; Antihypertensive activity; Angiotensin converting enzyme (ACE); Nitric oxide (NO); Endothelin-1 (ET-1)
资金
- Public Service Technology Application Research Project of Science and Technology Department of Zhejiang Province [LGN18D060002]
- National Natural Science Foundation of China [81673349]
In this report, eight antihypertensive peptides were isolated from protein hydrolysate of Antarctic krill (Euphausia superba) using ultrafiltration and chromatography consecutively, and their sequences were identified as Trp-Phe, Tyr-Arg-Lys-Glu-Arg, Tyr-Arg-Lys, Val-Asp, Tyr-Lys-Asp, Phe-Gln-Lys, Phe-Ala-Ser, and Phe-Arg-Lys-Glu. The IC50 values of Trp-Phe (0.32 +/- 0.05 mg/mL) and Phe-Ala-Ser (0.15 +/- 0.02 mg/mL) on ACE inhibitory activity were significantly (p <= .05) lower than those of the other six peptides. Furthermore, Trp-Phe, Tyr-Arg-Lys, Phe-Gln-Lys, and Phe-Ala-Ser did not only increase the nitric oxide (NO) concentration and decreased the content of endothelin-1 (ET-1) in the medium of human umbilical vein endothelial cells (HUVECs) in a dose dependent manner after 24 h, but also significantly reversed the decreased production of NO in the presence of 0.5 mu M norepinephrine and the effect of NE on ET-1 production. These results indicate that the isolated anti hypertensive peptides can correct the endothelial cell dysfunction induced by norepinephrine.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据