Dispersive liquid-liquid microextraction for rapid and inexpensive determination of tetramethylpyrazine in vinegar

The concentration of tetramethylpyrazine (TMP) in vinegar is an active indicator of vinegar quality. Dispersive liquid-liquid microextraction (DLLME) was first applied to vinegar as a clean-up pre-treatment for the rapid (5 min) determination of TMP by high-pressure liquid chromatography with ultraviolet detection (HPLC-UV), and may serve as an alternative to solid-phase extraction (SPE) or solid-phase microextraction (SPME). High sensitivity of HPLC for TMP determination was obtained using the DLLME pretreatment, with a limit of detection (LOD) of 0.001 mg L-1 and limit of quantification (LOQ) of 0.005 mg L-1. The developed method exhibited excellent linearity in the concentration range of 0.050-80.000 mg L-1, with a correlation coefficient R-2 > 0.999. Furthermore, the percentage recovery of TMP in vinegar using the developed method was within the range 97.97-105.24%. Therefore, DLLME coupled with HPLC-UV is a sensitive and promising method for vinegar clean-up and TMP assay.