期刊
FOOD CHEMISTRY
卷 282, 期 -, 页码 127-133出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.12.036
关键词
Glucosinolate; Brassica; LC-ESI-MS; Validation
资金
- World Institute of Kimchi - Ministry of Science and ICT, Republic of Korea [KE1803-3]
- National Research Council of Science & Technology (NST), Republic of Korea [KE1803-3] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Glucosinolates (GLS) have been reported to have significant anti-oxidative, antimicrobial, and anti-cancer activities. The current study was aimed to develop an analytical method for glucosinolate quantitation in eight Brassica species from Gwangju, Republic of Korea. For this purpose the UHPLC-Q-Orbitrap-MS technique was used and validated for optimal extraction conditions, detection and quantitation limits, linearity, precision, and accuracy. According to the results of GLS profiling, the total GLS concentration decreased in the order of cabbage > broccoli > cauliflower > mustard > kimchi cabbage > young radish similar to kale. All Brassica species contained glucoerucin (GER) and glucobrassicin (GBR) as major GLS with the high levels in cabbage (5.913 mu M/g) and broccoli (1.723 mu M/g), respectively. The contents of minor GLS were species-dependent, and could therefore be used for Brassica species classification.
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