Disposable Pipette Extraction (DPX) Coupled with Liquid Chromatography-Tandem Mass Spectrometry for the Simultaneous Determination of Pesticide Residues in Wine Samples

A novel multiresidue analytical method based on disposable pipette extraction (DPX) to determine the trace level of 28 pesticides in wine samples using liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed. The DPX method is used for both extraction and purification. After the optimization, targets were eluted with 1% HCl in acetonitrile and the eluent was placed into LC analysis. The developed method exhibited good linearity (R-2 > 0.99) for all 28 pesticides. Satisfactory recovery of 28 pesticides was observed ranging from 71 to 109%, with the intraday relative standard deviations (RSDs) and inter-day RSDs of 1-11% and 3-18%, respectively. Limits of detection (LODs) and limits of quantification (LOQs) were found to range from 1.5 to 3 mu g/L and 5 to 10 mu g/L, respectively. DPX was more suitable as compared with quick, easy, cheap, effective, rugged, and safe (QuEChERS) and solid-phase extraction (SPE) methods. The DPX method was applied to 47 commercial wine samples collected from China, and pesticide residues (0.02-0.09 mg/L) were found. In general, the developed method was highly reliable, selective, and sensitive for analyzing 28 pesticide residues in wine samples and would be applicable to other beverages.