Dummy Molecularly Imprinted Matrix Solid-Phase Dispersion for Selective Extraction of Seven Estrogens in Aquatic Products

A simple and sensitive method based on dummy molecular imprinting and matrix solid-phase dispersion (DMI-MSPD) was developed for the simultaneous detection of estrogens including estrone, 17 beta-estradiol, aestriol, ethinylestradiol, dienestrol, diethylstilbestrol, and hexestrol in aquatic products. The method was followed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). In this method, the dummy molecularly imprinted polymers (DMIPs) were synthesized by Pickering emulsion polymerization, in which genistein was used as template molecular. According to the results of the experiment, the method of DMI-MSPD showed high affinity and a good selectivity for the seven estrogens in aquatic products. Compared with conventional MSPD sorbents, including C18, Florisil, and silica gel, the DMIPs could not only selectively elute the seven estrogens from aquatic products but also reduce matrix disruptions. The mean recovery rates of seven estrogens ranged from 89 to 103%, with relative standard deviations less than 5%. The limits of detection (LOD) and limits of quantitation (LOQ) were 1.6-2.8 ng g(-1) and 5.4-7.6 ng g(-1), respectively. Therefore, the proposed method can be well applied to the detection of estrogens in aquatic products.