期刊
FEBS LETTERS
卷 593, 期 16, 页码 2250-2260出版社
WILEY
DOI: 10.1002/1873-3468.13490
关键词
capsaicin; cofilin; epithelial permeability; tight junction; TRPA1; TRPV4
资金
- Japan Society for Bioscience, Biotechnology, and Agrochemistry Funding Source: Medline
- Ministry of Education Culture and Sports (MEXT), Japan and Japan Science and Technology (JST) Funding Source: Medline
The transient receptor potential V4 channel (TRPV4) is responsive to a variety of physical and chemical stimuli, including a synthetic agonist GSK1016790A (GSK). Here, we show that TRPV4 is functionally expressed in, and that GSK induces the reversible opening of tight junctions (TJs) in epithelial Madin-Darby canine kidney II monolayers. Stimulation of TRPV4 by GSK induces an increase in fluorescein isothiocyanate-conjugated dextran (4 kDa) permeability and a reduction in transepithelial resistance, and these responses are blocked by pretreatment with the specific TRPV4 antagonist. Small conductance, but not large conductance Ca2+-activated K+ channels, TRPA1 channel, and cofilin activation are involved in TRPV4-mediated reversible opening of TJs. These results suggest that a novel mechanism underlies TRPV4-mediated regulation of the tightness of epithelial barriers.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据