期刊
FEBS LETTERS
卷 593, 期 19, 页码 2730-2741出版社
WILEY
DOI: 10.1002/1873-3468.13534
关键词
co-repressor; fatty acid synthesis; repression; transcription repressor
资金
- National Natural Science Foundation of China [31570728, 21573205]
- Anhui Provincial Natural Science Foundation [1808085QC84]
The Streptococcus pneumoniae fatty acid synthesis (FAS) pathway is globally controlled at the transcriptional level by the repressor FabT and its co-repressor acyl carrier protein (acyl-ACP), the intermediate of phospholipid synthesis. Here, we report the crystal structure of FabT complexed with a 23-bp dsDNA, which indicates that FabT is a weak repressor with low DNA-binding affinity in the absence of acyl-ACP. Modification of ACP with a long-chain fatty acid is necessary for the formation of a stable complex with FabT, mimicked in vitro by cross-linking, which significantly elevates the DNA-binding affinity of FabT. Altogether, we propose a putative working model of gene repression under the double control of FabT and acyl-ACP, elucidating a distinct repression network for Pneumococcus to precisely coordinate FAS.
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