期刊
EXPERIMENTAL HEMATOLOGY
卷 75, 期 -, 页码 21-25出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.exphem.2019.05.005
关键词
-
资金
- Intramural Research Program of the NHLBI
- NIDDK at the NIH
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [ZIAHL002338, ZIAHL006008] Funding Source: NIH RePORTER
Elevated fetal hemoglobin (HbF) is associated with reduced severity of sickle cell disease. Therefore, gamma-globin protein levels and F-cell (HbF-positive red blood cell) percentages are used for estimation of clinical benefit. Here, we monitored transplantation-related changes in HbF and F-cell percentages for rhesus macaques (Macaca mulatta) following total body irradiation or busulfan conditioning prior to CD34(+) cell transplantation. HbF protein expression peaked in the first 4-9 weeks posttransplant (0.99%-2.53%), and F-cells increased in the first 6-17 weeks posttransplant (8.7%-45.3%). HbF and F-cell ratios gradually decreased and stabilized to levels similar to those of control animals (1.96 +/- 1.97% for F cells and 0.49 +/- 0.19% gamma-globin expression) 4-7 months post-transplant. These findings confirm and expand on previous reports of transient induction in HbF and F-cell percentages in rhesus macaques following CD34+ cell transplantation, an observation that must be taken into consideration when evaluating therapeutic strategies that aim to specifically elevate HbF expression, which are currently in clinical development. Published by Elsevier Inc. on behalf of ISEH - Society for Hematology and Stem Cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据