Preparation of rosin-based polymer microspheres as a stationary phase in high-performance liquid chromatography to separate polycyclic aromatic hydrocarbons and alkaloids

Acrylpimaric acid ethylene glycol acrylate was used as the cross-linking agent to prepare rosin-based polymer microspheres through membrane emulsification-microsuspension polymerization. The prepared microspheres were used as the stationary phase in high-performance liquid chromatography for the separation of polycyclic aromatic hydrocarbons (PAHs) and two analogues of alkaloids. Five PAHs (benzene, naphthalene, fluorene, pyrene, and benzo(e)pyrene) were well separated in the rosin-based polymer column with the resolution (Rs) of 6.088 +/- 0.006, 5.759 +/- 0.017, 7.019 +/- 0.020, and 8.584 +/- 0.063. A linear relationship was observed for the carbon numbers of PAHs and lnK as obtained with the equation of ln k = 0.22n(c) - 2.45, R-2 = 0.996. The rosin-based polymer columns were also employed to separate theophylline-caffeine and camptothecin-10-hydroxy-camptothecin, and the good results were obtained with the Rs of 4.617 +/- 0.005 and 2.245 +/- 0.049, respectively.