期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 100, 期 23, 页码 10065-10080出版社
SPRINGER
DOI: 10.1007/s00253-016-7844-6
关键词
Binary imaging; Bioreactor cascade; Cupriavidus necator; Footprint area analysis; PHA granules; Poly[3-(R)-hydroxybutyrate] (PHB)
资金
- University of Graz (Austria)
- European Commission [245084]
- BASF SE by financing the projects Evaluation of strains and process for the production of PHA-step 1 and 2.
Statistical distribution of cell and poly[3-(R)-hydroxybutyrate] (PHB) granule size and number of granules per cell are investigated for PHB production in a five-stage cascade (5CSTR). Electron microscopic pictures of cells from individual cascade stages (R1-R5) were converted to binary pictures to visualize footprint areas for polyhydroxyalkanoate (PHA) and non-PHA biomass. Results for each stage were correlated to the corresponding experimentally determined kinetics (specific growth rate mu and specific productivity pi). Log-normal distribution describes PHA granule size dissimilarity, whereas for R1 and R4, gamma distribution best reflects the situation. R1, devoted to balanced biomass synthesis, predominately contains cells with rather small granules, whereas with increasing residence time tau, maximum and average granule sizes by trend increase, approaching an upper limit determined by the cell's geometry. Generally, an increase of intracellular PHA content and ratio of granule to cell area slow down along the cascade. Further, the number of granules per cell decreases with increasing tau. Data for mu and pi obtained by binary picture analysis correlate well with the experimental results. The work describes long-term continuous PHA production under balanced, transient, and nutrient-deficient conditions, as well as their reflection on the granules size, granule number, and cell structure on the microscopic level.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据