期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 100, 期 22, 页码 9641-9652出版社
SPRINGER
DOI: 10.1007/s00253-016-7763-6
关键词
Actinobacillus succinogenes; Biofilm; Glucose-6-phosphate dehydrogenase; Metabolic flux analysis; Pentose phosphate pathway; Succinic acid
资金
- National Research Foundation (NRF)
Increased pentose phosphate pathway flux, relative to total substrate uptake flux, is shown to enhance succinic acid (SA) yields under continuous, non-growth conditions of Actinobacillus succinogenes biofilms. Separate fermentations of glucose and xylose were conducted in a custom, continuous biofilm reactor at four different dilution rates. Glucose-6-phosphate dehydrogenase assays were performed on cell extracts derived from in situ removal of biofilm at each steady state. The results of the assays were coupled to a kinetic model that revealed an increase in oxidative pentose phosphate pathway (OPPP) flux relative to total substrate flux with increasing SA titre, for both substrates. Furthermore, applying metabolite concentration data to metabolic flux models that include the OPPP revealed similar flux relationships to those observed in the experimental kinetic analysis. A relative increase in OPPP flux produces additional reduction power that enables increased flux through the reductive branch of the TCA cycle, leading to increased SA yields, reduced by-product formation and complete closure of the overall redox balance.
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