期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 100, 期 16, 页码 7037-7049出版社
SPRINGER
DOI: 10.1007/s00253-016-7397-8
关键词
Monascus pigment; Pigment intermediate; Biosynthesis pathway; Monascus ruber
资金
- Major Program of National Natural Science Foundation of China [31330059]
- National Natural Science Foundation of China [31271834, 31171649, 31371824]
Monascus pigments (Mps) are a group of azaphilonic secondary metabolites produced by Monascus spp. via a polyketide pathway. A mutant deleted an about 30 kb region of Mps gene cluster from Monascus ruber M7 was isolated previously, which produces a high amount of a light yellow pigment. The current study revealed that the mutant named Delta MpigJ-R lost proximate eight genes of the Mps gene cluster in M. ruber M7 through genetic analysis at DNA and RNA levels. The produced light yellow material was identified as a benzaldehyde derivative named as 6-(4-hydroxy-2-oxopentyl)-3-methyl-2, 4-dioxocyclohexane carb-aldehyde (M7PKS-1) by FT-IR, NMR, and MS. The sodium acetate-1-C-13 feeding experiment indicated that M7PKS-1 was a product produced from polyketide pathway. Finally, the feeding of M7PKS-1 helped to induce and regain Mps production of the mutants (Delta MpigA and Delta MpigE) which were previously unable to biosynthesize Mps and proved that M7PKS-1 was an initial intermediate of Mps. The results in this study provide a line of action to unveil Monascus pigments biosynthesis pathway.
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