期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 100, 期 5, 页码 2097-2105出版社
SPRINGER
DOI: 10.1007/s00253-015-7276-8
关键词
Amino acids; Novel technologies; Synthetic biology; Microorganisms
资金
- National Natural Science Foundation of China [31370085]
- China Postdoctoral Science Foundation [2014 M551894]
- Special Funds of Shandong Postdoctoral Innovation Project [201402026]
Traditionally, amino acid-producing strains were obtained by random mutagenesis and subsequent selection. With the development of genetic and metabolic engineering techniques, various microorganisms with high amino acid production yields are now constructed by rational design of targeted biosynthetic pathways. Recently, novel technologies derived from systems and synthetic biology have emerged and open a new promising avenue towards the engineering of amino acid production microorganisms. In this review, these approaches, including rational engineering of rate-limiting enzymes, real-time sensing of end-products, pathway optimization on the chromosome, transcription factor-mediated strain improvement, and metabolic modeling and flux analysis, were summarized with regard to their application in microbial amino acid production.
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