期刊
DEVELOPMENTAL NEUROSCIENCE
卷 41, 期 1-2, 页码 79-93出版社
KARGER
DOI: 10.1159/000499364
关键词
Oligodendrocyte progenitor cells; Oligodendrocytes; Neural stem cells; Neuroepithelial stem cells; Neural glial differentiation
资金
- National Key Research & Development Program
- National Natural Science Foundations of China [2017YFC1200204, 31670171]
- Neural Regeneration Co-Innovation Center of Jiangsu Province
- Basic Research of Jiangsu Education Department [12KJA3100031]
- Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions and Nantong Science and Technology grant [MS12015055]
- student innovation project of Central South University [2018zzts231]
Oligodendrocyte progenitor cells (OPCs) may have beneficial effects in cell replacement therapy of neurodegenerative disease owing to their unique capability to differentiate into myelinogenic oligodendrocytes (OLs) in response to extrinsic signals. Therefore, it is of significance to establish an effective differentiation methodology to generate highly pure OPCs and OLs from some easily accessible stem cell sources. To achieve this goal, in this study, we present a rapid and efficient protocol for oligodendroglial lineage differentiation from mouse neural stem cells (NSCs), rat NSCs, or mouse embryonic stem cell-derived neuroepithelial stem cells. In a defined culture medium containing Smoothened Agonist, basic fibroblast growth factor, and platelet-derived growth factor-AA, OPCs could be generated from the above stem cells over a time course of 4-6 days, achieving a cell purity as high as similar to 90%. In particular, these derived OPCs showed high expandability and could further differentiate into myelin basic protein-positive OLs within 3 days or alternatively into glial fibrillary acidic protein-positive astrocytes within 7 days. Furthermore, transplantation of rodent NSC-derived OPCs into injured spinal cord indicated that it is a feasible strategy to treat spinal cord injury. Our results suggest a differentiation strategy for robust production of OPCs and OLs from rodent stem cells, which could provide an abundant OPC source for spinal cord injury.
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