期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 95, 期 -, 页码 28-37出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2019.01.013
关键词
Crassostrea gigas; SPRY domain-containing SOCS box protein 3 (SPSB3); Immune response; Granulocytes; Cytokine production
资金
- National Key RD Program [2018YFD0900304]
- NSFC [U1706204]
- Key R&D Program of Liaoning Province [201703148]
- Modern Agro-industry Technology Research System [CARS-49]
- Fund for Outstanding Talents and Innovative Team of Agricultural Scientific Research
- AoShan Talents Cultivation Program - Qingdao National Laboratory for Marine Science and Technology [2017ASTCP-0513]
- Dalian High Level Talent Innovation Support Program [2015R020]
- Research Foundation for Distinguished Professor in Liaoning
- Program for Innovative Talents in Higher Education of Liaoning Province [LR2016036]
- Talented Scholars in Dalian Ocean University
The sp1A/ryanodine receptor (SPRY) family members have been reported to involve in important biological pathways, including innate immune signaling, cytokine signaling suppression, development, cell growth, and retroviral restriction. In the present study, a SPRY domain-containing SOCS box protein (named as CgSPSB3) was identified and characterized from oyster Crassostrea gigas. The open reading frame of CgSPSB3 gene was of 699 bp, encoding a polypeptide of 232 amino acid residues with a central SPRY domain and a C-terminal SOCS box motif. CgSPSB3 mRNA transcripts could be detected in all the examined tissues with the highest level in hemocytes, which was about 82.72-fold (p < 0.05) of that in gonad. Furthermore, the expression level of CgSPSB3 mRNA in granulocytes was significantly higher than that in semi-granulocytes and agranulocytes, which was about 2.04-fold (p < 0.05) of the average level of hemocytes. Immunofluorescence assay further revealed that CgSPSB3 protein was mainly distributed in the cytoplasm of granulocytes. The mRNA expression of CgSPSB3 in hemocytes was up-regulated after lipopolysaccharide (LPS) and Vibrio splendidus stimulations. The mRNA expression of CgIFNLP, CgIL17-5 and CgTNF-1 decreased significantly (p < 0.05) at 24 h after the CgSPSB3 mRNA was knocked down by RNAi. These results collectively indicated that CgSPSB3 might play an important role in regulating cytokines production in granulocytes of C. gigas.
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