4.8 Article

The Bub1-TPR Domain Interacts Directly with Mad3 to Generate Robust Spindle Checkpoint Arrest

期刊

CURRENT BIOLOGY
卷 29, 期 14, 页码 2407-+

出版社

CELL PRESS
DOI: 10.1016/j.cub.2019.06.011

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资金

  1. Darwin Trust of Edinburgh
  2. Wellcome Trust [202811, 108105]
  3. Wellcome Centre for Cell Biology core grants [092076, 203149]
  4. Medical Research Council [MR/K501293/1]
  5. NIH [GM064386]

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The spindle checkpoint monitors kinetochore-microtubule interactions and generates a wait anaphase delay when any defects are apparent [1-3]. This provides time for cells to correct chromosome attachment errors and ensure high-fidelity chromosome segregation. Checkpoint signals are generated at unattached chromosomes during mitosis. To activate the checkpoint, Mps1(Mph1) kinase phosphorylates the kinetochore component KNL1(Spc105/Spc7) on conserved MELT motifs to recruit Bub3-Bub1 complexes [4-6] via a direct Bub3 interaction with phospho-MELT motifs [7, 8]. Mps1(Mph1) then phosphorylates Bub1, which strengthens its interaction with Mad1-Mad2 complexes to produce a signaling platform [9, 10]. The Bub1-Mad1 platform is thought to recruit Mad3, Cdc20, and Mad2 to produce the mitotic checkpoint complex (MCC), which is the diffusible wait anaphase signal [9, 11, 12]. The MCC binds and inhibits the mitotic E3 ubiquitin ligase, known as Cdc20-anaphase promoting complex/cyclosome (APC/C), and stabilizes securin and cyclin to delay anaphase onset [13-17]. Here we demon-strate, in both budding and fission yeast, that kinetochores and KNL1(Spc105/Spc7) can be bypassed; simply inducing heterodimers of Mps1(Mph1) kinase and Bub1 is sufficient to trigger metaphase arrest that is dependent on Mad1, Mad2, and Mad3. We use this to dissect the domains of Bub1 necessary for arrest, highlighting the need for Bub1-CD1, which binds Mad1 [9], and Bub1's highly conserved N-terminal tetratricopeptide repeat (TPR) domain [18, 19]. We demonstrate that the Bub1 TPR domain is both necessary and sufficient to bind and recruit Mad3. We propose that this brings Mad3 into close proximity to Mad1-Mad2 and Mps1(Mph1) kinase, enabling efficient generation of MCC complexes.

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