期刊
BIOMATERIALS
卷 48, 期 -, 页码 56-65出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2015.01.020
关键词
Layer-by-layer assembling; Instructive surfaces; Platelet lysate; Growth factors; Cell behavior; Platelet derivative
资金
- Portuguese Foundation for Science and Technology [SFRH/BD/70107/2010]
- European Union [REGPOT-CT2012-316331-POLARIS, FP7-KBBE-2010-4-266033 - SPECIAL]
- European Research Council [ERC-2012-ADG 20120216-321266]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/70107/2010] Funding Source: FCT
Great efforts have been made to introduce growth factors (GFs) onto 2D/3D constructs in order to control cell behavior. Platelet lysate (PL) presents itself as a cost-effective source of multiple GFs and other proteins. The instruction given by a construct-PL combination will depend on how its instructive cues are presented to the cells. The content, stability and conformation of the GFs affect their instruction. Strategies for a controlled incorporation of PL are needed. Herein, PL was incorporated into nanocoatings by layer-by-layer assembling with polysaccharides presenting different sulfation degrees (SD) and charges. Heparin and several marine polysaccharides were tested to evaluate their PL and GF incorporation capability. The consequent effects of those multilayers on human adipose derived stem cells (hASCs) were assessed in short-term cultures. Both nature of the polysaccharide and SD were important properties that influenced the adsorption of PL, vascular endothelial growth factor (VEGF), fibroblast growth factor b (FGFb) and platelet derived growth factor (PDGF). The sulfated polysaccharides-PL multilayers showed to be efficient in the promotion of morphological changes, serum-free adhesion and proliferation of high passage hASCs (P > 5). These biomimetic multilayers promise to be versatile platforms to fabricate instructive devices allowing a tunable incorporation of PL. (C) 2015 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据