期刊
CHEMICO-BIOLOGICAL INTERACTIONS
卷 306, 期 -, 页码 152-162出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2019.05.003
关键词
Punicalagin; Myocardial ischemia/reperfusion; SIRT1; Oxidative stress; Nitrosative stress; Inflammatory response
资金
- National Natural Science Foundation of China [81700264, 81570232]
- Postdoctoral Science Foundation Grant of China [2018M643839]
- Natural Science Foundation of Liaoning Province [20170540973]
Punicalagin has been found to exert cardiac protective effects against myocardial ischemia/reperfusion (MI/R) injury, although the detailed mechanisms remain largely unknown. This experiment was performed to explore the potential involvement of silent information regulator 1 (SIRT1)-NFE2-related factor 2 (NRF-2)-heme oxygenase-1 (HO-1) pathway in the cardiac protective actions of punicalagin. Sprague-Dawley (SD) rats were subjected to MI/R operation with or without punicalagin treatment (40 mg kg(-1)d(-1)). We showed that punicalagin-treated group exhibited enhanced cardiac function, reduced myocardial infarction and decreased cleaved caspase-3 level. Furthermore, myocardial oxidative/nitrosative stress was ameliorated by punicalagin as evidenced by suppressed superoxide generation, gp91(phox) and iNOS expressions, NO metabolites as well as myocardial nitrotyrosine level. Additionally, punicalagin decreased myocardial IL-6, TNF-alpha and the levels of ICAM-1, VCAM-1 and IKK-beta expressions as well as I kappa B-alpha phosphorylation and NF-kappa B nuclear translocation. However, these effects were abolished by EX527 (5 mg kg(-1)d(-1), a selective SIRT1 inhibitor). We further found that punicalagin dose-dependently enhanced SIRT1 nuclear distribution and NRF-2-HO-1 signaling. While EX527 treatment not only reduced SIRT1 activity, but also reversed the activation of NRF-2-HO-1 pathway. Collectively, these results revealed that punicalagin reduced cardiac oxidative/nitrosative stress and inflammatory response induced by MI/R operation through SIRT1-mediated activation of NRF-2-HO-1 signaling.
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