期刊
CARBOHYDRATE POLYMERS
卷 214, 期 -, 页码 71-79出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2019.03.004
关键词
Agrimonia pilosa; Polysaccharide; Steroid induced avascular necrosis of the femoral head (SANFH); Osteoblast; Apoptosis; Dexamethasone
资金
- National Natural Science Foundation of China [81672166]
In this study, one homogeneous polysaccharide (APP-AW), with an average molecular weight of 9550 Da, was purified from Agrimonia pilosa. Analysis by gas chromatography (GC), methylation, UV, Infrared spectra (IR), 1D and 2D nuclear magnetic resonance (NMR) spectroscopy indicated that APP-AW was a beta-(1 -> 3)-D-glucan. The effect of APP-AW on dexamethasone (Dex)-induced apoptosis in osteoblasts was also examined. Pretreatment of APP-AW (100 mu g/ml) significantly attenuated cell loss and apoptosis induced by Dex (1 mu M) in osteoblasts as determined by MTT, Annexin V-FITC/propidium iodide (PI) and Transferase (TdT)-mediated dUTP nick end labeling (TUNEL) staining assay. Collectively, the present study demonstrated that APP-AW might be an alternative therapeutics for the treatment of SANFH via reducing Dex-induced bone cellular apoptosis.
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