期刊
CANCER LETTERS
卷 452, 期 -, 页码 79-89出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2019.02.044
关键词
CAFs; Autocrine TGF-beta 1; DNMT3B; miR-200s; miR-221
类别
资金
- National Natural Science Foundation of China [NSFC 81773078, NSFC 81402180, NSFC 81472476, NSFC 31671481, NSFC 81802653]
- First Affiliated Hospital of Chongqing Medical University [PYJJ2018-04]
Cancer-associated fibroblasts (CAFs) remain active even in the absence of cancer cells. However, the molecular mechanism underlying the sustained active status of CAFs is largely unrevealed. We found that in CAFs, DNMT3B was not only a target of miR-200b, miR-200c and miR-221, but was able to induce DNA methylation of miR-200s promoters. DNMT3B eventually reached a stably high level by the counteracting effect of decreasing miR-200b/c and increasing miR-221 in normal fibroblasts (NFs) with long-term exogenous TGF-beta 1 treatment, and DNMT3B further led to a low level of miR-200s which established CAF activation. Meanwhile, miR-200s/miR-221/DNMT3B signaling sustained autocrine TGF-beta 1 maintaining active CAF status. Destruction of the autocrine TGF-beta 1/miR-200s/miR-221/DNMT3B signaling led to demethylation of miR-200s promoters and further restored the NF phenotypes. Moreover, we confirmed that TCF12, the target of miR-141, stimulated c-Myc/Cyclin D1 axis in breast cancer cells to promote cancer growth by enhancing CXCL12 of CAFs. The current study reveals that the TGF-beta 1/miR-200s/miR-221/DNMT3B regulatory loop is responsible for the maintenance of CAFs status and is also necessary for CAF function in promoting malignance of breast cancer, which provides a potential target for CAF-driven therapeutic strategy.
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