期刊
CANCER LETTERS
卷 454, 期 -, 页码 179-190出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2019.03.052
关键词
Colorectal cancer; Cervical cancer; H3K4 methylation; Chemotherapy sensitivity
类别
资金
- Project of the National Natural Science Foundation of China [81773198]
- National Keypoint Research and Invention Program of the China Ministry of Science and Technology [MOST-2016YFC1303200]
- National ST Major project [2018ZX09201018]
Lysine-specific demethylasel (LSD1) plays a crucial role in cancer and has become a promising target for cancer therapy. However, the mechanism underlying the role of LSD1 in oncogenesis is poorly understood, and more effective LSD1 inhibitors are needed. Here we report the biological activity of a novel LSD1 inhibitor named ZY0511. ZY0511 specifically inhibited LSD1 activity and the proliferation of various human cancer cells especially the HeLa and HCT116 cells. ZY0511 significantly increased the expression of DDIT4, a known mTORC1 suppressor, which was a direct downstream target of LSD1 confirmed by ChIP-PCR. ZY0511-induced LSD1 inhibition upregulated the expression of DDIT4 by altering histone H3K4 methylation levels at its promoter, thus suppressing mTORC1 activity. Knockdown of DDIT4 attenuated the anticancer effect of ZY0511. Intraperitoneal administration of ZY0511 significantly prevented the growth of HCT116 and HeLa xenografts in mice and showed no detectable toxicity. Moreover, DDIT4 expression was correlated with the sensitivity of human cancer cells to chemotherapy. Taken together, ZY0511 showed therapeutic potential for solid tumors, the induction of DDIT4 may be used as a predictive biomarker of LSD1 inhibitors.
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