期刊
CANCER LETTERS
卷 454, 期 -, 页码 26-36出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2019.03.055
关键词
AML; Apoptosis; BCL-2; H22954; Long non-coding RNA
类别
资金
- National Science Foundation of China, China [81170468, 81302382]
- National Key Scientific Projects of China, China [2011CB933501]
- Natural Scientific Foundation of Jiangsu Scientific Bureau, China [BK2011266]
- Priority Academic Program Development of Jiangsu Higher Education Institutions, China
- High-level talents in six industries of Jiangsu, China [WSN-066]
- 2012 Jiangsu Provincial Special Program of Medical Science, China [BL2012005]
- Jiangsu Key Medical Center, China [ZX201102]
Long non-coding RNAs (lncRNAs) are important in cancer biology. In this study, we analyzed differentially expressed genes in CD34(+) hematopoietic cells and identified a novel IncRNA, H22954, which was down regulated in acute myeloid leukemia (AML) patients. In cultured AML cells and mouse xenograft models, H22954 expression inhibited cell proliferation and tumor growth, respectively. Bioinformatic analysis and RNA antisense purification assay indicated that H22954 targeted the 3' untranslated region of the BCL2 gene. In luciferase assays, H22954 expression inhibited BCL2 expression. In transfected K562 cells and mouse xenograft tumors, H22954 overexpression reduced BCL-2 protein levels and promoted cell death. In AML patients, H22954 expression inversely correlated with BCL-2 protein levels in bone marrow cells, blast cell numbers and disease prognosis. These results indicate that H22954 is a novel regulator of BCL-2 and that reduced H22954 expression may play an important role in the pathogenesis of AML.
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