期刊
BIOMATERIALS
卷 54, 期 -, 页码 213-225出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2015.02.079
关键词
Hydrogel; Mesenchymal stem cell; Biodegradation; Bioactivity; Biomimetic material; Cartilage tissue engineering
资金
- Australian Commonwealth Scientific and Industrial Research Organisation (CSIRO) in the Manufacturing Flagship
- Medical Engineering Solutions in Osteoarthritis Centre of Excellence - Wellcome Trust [088844]
- UK Regenerative Medicine Platform Hub Acellular Approaches for Therapeutic Delivery - Medical Research Council [MR/K026682/1]
- Engineering and Physical Sciences Research Council
- Biotechnology and Biological Sciences Research Council
- ERC
- Wellcome Trust Senior Investigator Award [098411/Z/12/Z]
- Medical Research Council [MR/K026682/1] Funding Source: researchfish
- MRC [MR/K026682/1] Funding Source: UKRI
- Wellcome Trust [098411/Z/12/Z] Funding Source: Wellcome Trust
Regenerative medicine strategies for restoring articular cartilage face significant challenges to recreate the complex and dynamic biochemical and biomechanical functions of native tissues. As an approach to recapitulate the complexity of the extracellular matrix, collagen-mimetic proteins offer a modular template to incorporate bioactive and biodegradable moieties into a single construct. We modified a Streptococcal collagen-like 2 protein with hyaluronic acid (HA) or chondroitin sulfate (CS)-binding peptides and then cross-linked with a matrix metalloproteinase 7 (MMP7)-sensitive peptide to form biodegradable hydrogels. Human mesenchymal stem cells (hMSCs) encapsulated in these hydrogels exhibited improved viability and significantly enhanced chondrogenic differentiation compared to controls that were not functionalized with glycosaminoglycan-binding peptides. Hydrogels functionalized with CS-binding peptides also led to significantly higher MMP7 gene expression and activity while the HA-binding peptides significantly increased chondrogenic differentiation of the hMSCs. Our results highlight the potential of this novel biomaterial to modulate cell-mediated processes and create functional tissue engineered constructs for regenerative medicine applications. (C) 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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