期刊
BIOMATERIALS
卷 53, 期 -, 页码 12-24出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2015.02.075
关键词
Cell encapsulation; In vivo cell tracking; Cell therapy; Myocardial infarction; Biocompatibility; Foreign body response
资金
- Joint Council Office under the Agency for Science, Technology and Research (A*STAR), Singapore [JCO 10/03/FG/06/05]
- Life Sciences Institute at NUS
- NUS Graduate School of Engineering and Integrative Sciences (NGS)
- scholarship SINGA Singapore International Graduate Award (A*STAR, NUS)
The limited efficacy of cardiac cell-based therapy is thought to be due to poor cell retention within the myocardium. Hence, there is an urgent need for biomaterials that aid in long-term cell retention. This study describes the development of injectable microcapsules for the delivery of mesenchymal stem cells (MSCs) into the infarcted cardiac wall. These microcapsules comprise of low concentrations of agarose supplemented with extracellular matrix (ECM) proteins collagen and fibrin. Dextran sulfate, a negatively charged polycarbohydrate, was added to mimic glycosaminoglycans in the ECM. Cell viability assays showed that a combination of all components is necessary to support long-term survival and proliferation of MSCs within microcapsules. Following intramyocardial transplantation, microcapsules degraded slowly in vivo and did not induce a fibrotic foreign body response. Pre-labeling of encapsulated MSCs with iron oxide nanoparticles allowed continued cell-tracking by MRI over several weeks following transplantation into infarcted myocardium. In contrast, MSCs injected as cell suspension were only detectable for two days post transplantation by MRI. Histological analysis confirmed integration of transplanted cells at the infarct site. Therefore, microcapsules proved to be suitable for stem cell delivery into the infarcted myocardium and can overcome current limitations of poor cell retention in cardiac cell-based therapy. (C) 2015 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据