期刊
BIOTECHNOLOGY LETTERS
卷 41, 期 8-9, 页码 1033-1041出版社
SPRINGER
DOI: 10.1007/s10529-019-02698-y
关键词
Actinomycete; Biosynthesis; Marinacarboline A; NfRibA; Nocardiopsis flavescens; Riboflavin; Secondary metabolite
资金
- NSFC [41806158, 41406195]
- Provincial Natural Science Research Project of Anhui Colleges and Universities [KJ2017A177]
- Key Laboratory of Tropical Marine Bio-resources and Ecology, SCSIO, CAS [2018011007]
- National Training Program of Innovation and Entrepreneurship for Undergraduates [201710366031]
- Natural Science Foundation of Zhejiang Province [LY17C160001]
- research project of Education Department of Zhejiang Province [Y201533256]
- research project of Science Technology Department of Zhejiang Province [2016C37036]
- Open Project Program of Guangdong Key Laboratory of Marine Materia Medica, SCSIO, CAS [2018011007]
ObjectivesTo improve the bioproductivity of secondary metabolites of marine derived Nocardiopsis flavescens CGMCC 4.5723 by enhancing its riboflavin supplement.ResultsThe NfRibA, type II guanosine triphosphate (GTP) cyclohydrolase (GCH II) of Nocardiopsis flavescens CGMCC 4.5723, was biochemically identified and showed that NfRibA could efficiently catalyze the first step of riboflavin biosynthesis to hydrolyze GTP into 2, 5-diamino-6-ribosylamino-4(3H)-pyrimidinedione 5-phosphate (DARPP) with K-m value of 160.11 +/- 26.81 mu M in vitro. The overexpression of NfribA could obviously increase riboflavin bioproduction to the titers of 0.41 +/- 0.19mg/l by comparing with the wild type counterpart. Consequently, this rise of riboflavin bioproduction did not disturb the expression of genes involved in marinacarboline A biosynthesis, but could significantly enhance its bioproduction with the titer of 5.5 +/- 0.17mg/l through comparing with wild type control.Conclusions Optimization of riboflavin supplement could be a new promising strategy in actinomycetic marinacarboline A exploitation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据