期刊
BIOTECHNOLOGY JOURNAL
卷 14, 期 10, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201900215
关键词
enzymes; ionic liquids; lipases; recycling; thermomorphic solvent (TMS)-systems; two-phase system
资金
- Deutsche Forschungsgemeinschaft [KR 2491/11-1] Funding Source: Medline
- DFG [KR 2491/11-1] Funding Source: Medline
- German Federal Ministry of Education and Research [031A123] Funding Source: Medline
- Bundesministerium für Bildung und Forschung [031A123] Funding Source: Medline
- Leibniz ScienceCampus Phosphorus Research Rostock Funding Source: Medline
The applicability of ionic liquid-water-based thermomorphic solvent (TMS)-systems with an upper critical solution temperature for homogeneous biocatalysis is investigated. Cholinium- and imidazolium-based ionic liquids are used to facilitate a temperature-dependent phase change, which can be easily fine-tuned by adding salts or polar organic solvents. Within the TMS-system, a high enzymatic activity and subsequent full conversion is achieved in the intermittent monophasic reaction system of the TMS-system. Therefore, the biocatalyst can be easily recycled after separating the phases at lower temperatures.
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