期刊
BIOSENSORS & BIOELECTRONICS
卷 137, 期 -, 页码 123-132出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.05.012
关键词
Biosensors; Aptamers; Nanoparticles; Interleukin-6; Label-free detection
类别
资金
- Romanian National Authority for Scientific Research and Innovation, CNCS/CCCDI-UEFISCDI, within PNCDI III [PN-III-P1-1.2-PCCDI2017-0221]
- Romanian National Authority for Scientific Research and Innovation (ANCSI) [PN18-03 02 01]
Despite the fact that cancer research has experienced important advances and remarkable improvement in the curing processes during the last decades, this disease still occupies a leading position among the causes of death worldwide. It has been demonstrated that there is an interconnection between the overexpression of interleukin-6 cytokine and the tumor growth, metastasis, and therapeutic resistance in several types of malignancies. Herein, a highly sensitive and selective aptasensor for quantitative detection of interleukin-6 was developed by using a glassy carbon electrode modified with p-aminobenzoic acid, p-aminothiophenol and gold nanoparticles. A thio-terminated aptamer specific for interleukin-6 was immobilized on the surface of the modified electrode via the formation of gold-sulfur bonds. This DNA oligonucleotide was then used as a detection probe to capture the target protein at the biosensor surface allowing label-free detection by electrochemical impedance spectroscopy. The developed aptasensor showed a good linear response from 5 pgmL(-1) to 100 ngmL(-1) with a detection limit of 1.6 pgmL(-1), within the range of physiological concentration of the protein. The biosensor exhibited high selectivity and has been successfully used to detect interleukin-6 in blood samples collected from patients suffering of colorectal cancer, with excellent recoveries after the addition of known amount of the target protein.
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