期刊
BIOSENSORS & BIOELECTRONICS
卷 135, 期 -, 页码 82-87出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.04.007
关键词
Electrochemical analysis; Glucose biosensor; Cancer cell membrane; Glucose transporter-1; Permselectivity
类别
资金
- National Research Foundation of Korea Grant - Korean Government (MSIP) [NRF-2016R1A2B4010269, NRF-2017R1A6A3A11034311, NRF-2018M3C1B7020722]
- Ministry of Trade, Industry and Energy under Industrial Technology Innovation Program [10079316]
- Korea University Grant
Enzymatic blood glucose detection with selectivity is one of the most important conundrums, because human blood contains many components that can hinder enzyme-substrate reactions. Meanwhile, cancer cells express much higher levels of glucose transporter-1 on their cell membrane to selectively and excessively uptake more alpha-D-glucose than do normal cells. Inspired by such cellular permselectivity for glucose, herein we significantly improved the selectivity of a glucose sensor by using a breast cancer cell membrane (BCCM). The BCCM was extracted from MDA-MB-231 cells and coated onto an enzyme-deposited electrode via a vesicle fusion method. We investigated BCCM-coated sensors using ATR-FTIR, SEM, AFM, and cyclic voltammetry. The exceptional permselectivity of BCCM-coated sensors was validated using glucose solutions containing various interfering molecules (e.g., D-( - )-fructose, D-( + )-xylose, D-( + )-maltose, L-cysteine, L-ascorbic acid, and uric acid) and human serum (4.35-7.35 mM of glucose), implying their high potential for practical use.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据