期刊
BIOSENSORS & BIOELECTRONICS
卷 137, 期 -, 页码 154-160出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.05.003
关键词
Paper-based microfluidics; Signal amplification; Point-of-care biosensor; Glucometer; Ag+
类别
资金
- National Science Foundation of China [21765007, 21565012, 21365009, 21874032]
- Guangxi Key Research Project [GuikeAB17129003]
- Guangxi Science Fund for Distinguished Young Scholars [2018GXNSFFA281002]
- Open Foundation of Guangxi Key Laboratory of Electrochemical and Magnetochemical Functional Materials [EMFM20181120]
A variety of routine methods are available for the detection of silver (I) (Ag+) ions, but most of them rely on expensive, sophisticated and desktop instruments. Herein, a low-cost, instrument-free and portable Ag+ biosensor was described by initially designing a new class of 3D origami microfluidic paper-based analytical devices (mu PADs) into each of which one piece of reagent-loaded nanoporous membrane was integrated. It combines analyte-triggered self-growing of silver nanoparticles to block the membranes pores in situ for rapid yet efficient signal amplification with a handheld personal glucose meter for a portable and sensitive quantitative readout based on the biocatalytic reactions between the glucose oxidase and glucose. Its utility is well demonstrated with the specific detection of the analyte with a limit of detection as low as similar to 58.1 pM (3 sigma), which makes this new biosensing method one of the most sensitive Ag+ assays in comparison with many other typical methods recently reported. Moreover, the satisfactory recovery of analyzing several types of real water examples, i.e., tap water, drinking water, pond water and soil water, additionally validates its feasibility for practical applications.
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