期刊
BIOSENSORS & BIOELECTRONICS
卷 135, 期 -, 页码 173-180出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.04.023
关键词
Aggregation-induced emission; Fluorescent microsphere; Escherichia coli O157:H7; Immunochromatographic assay
类别
资金
- National Natural Science Foundation of China [31772066]
- Open Project Program of State Key Laboratory of Food Science and Technology, Nanchang University [SKLF-KF-201616]
- Jiangxi earmarked fund for Jiangxi Agriculture Research System [JXARS-03]
Fluorescent microsphere (FM) is widely used as probe in immunochromatographic assay (ICA). However, the performance of conventional FM is limited because of the aggregation-caused quenching effect. Herein, we compared a kind of conventional FM (DMFFM, loading DMF) with novel aggregation-induced emission FM (AIEFM, loading TCBPE). The fluorescence intensity of DMFFM initially increased and then decreased as the concentrations of the loading DMF increased. The fluorescence intensity of AIEFM increased as the concentrations of the loading TCBPE increased and retained a high value. AIEFM was compared with two commercial FMs purchased from Ocean (OFM) and Merk (MFM). The maximum fluorescence intensity and relative quantum yield of AIEFM was approximately 5 and 4.5 times higher than those of two commercial FMs. We used the novel AIEFM as a probe to improve the sensitivity of ICA. When Escherichia coli O157:H7 was detected as the target, the limit of detection of ICA based on AIEFM, OFM and MFM were 3.98 x 10(3) CFU/mL, 4.48 x 10(4) and 2.78 x 10(4) CFU/mL, respectively. The ICA of AIEFM had 11 and 7 times improvement in sensitivity compared with that of OFM and MFM. Our results could be used as a basis for novel probes in practical ICA applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据