期刊
BIOSENSORS & BIOELECTRONICS
卷 135, 期 -, 页码 8-13出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.04.004
关键词
ZnO as coreaction accelerator; Dissolved O-2 as coreactant; miRNA-21 detection; DNA dendrimer
类别
资金
- NNSF of China [21775124, 21575116, 21675129]
- Fundamental Research Funds for the Central Universities [XDJK2018AA0003]
In this work, the ZnO nanostars with excellent catalytic performance were firstly used as the coreaction accelerator of luminol-O-2 system to construct a biosensor for ultrasensitively detecting microRNA-21 (miRNA-21) in cancer cells. Specifically, ZnO nanostars could expedite the reduction of dissolved O-2, generating more reactive oxygen species (ROSs) to extremely promote electrochemiluminescence (ECL) luminous efficiency of luminol. Thus luminol-functionalized Au NPs@ZnO (L-Au NPs@ZnO) nanomaterials were employed as signal probe to fabricate sensing nano-platform for achieving significant ECL emission as signal on state. Moreover, upon the addition of a tiny minority of target miRNA-21, massive ferrocene (Fc) could be immobilized on the sensing interface through hybridization chain reaction (HCR) triggered-DNA dendrimers self-assembly, in which Fc consumed dissolved O-2 for prominently quenching the ECL emission of signal probe and then reached a signal off state. As a result, the biosensor performed a good linearity in 100 aM - 100 pM and a low limit of detection (LOD) down to 18.6 aM. In general, this work utilized a new coreaction accelerator as an efficient amplification approach for ultrasensitively detecting target analyses, providing a promising approach in luminol-centric ECL bioanalysis fields.
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