期刊
BIORESOURCE TECHNOLOGY
卷 281, 期 -, 页码 239-249出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.02.082
关键词
Enzymatic hydrolysis; Lipids; Pig manure; Pretreatment; Proteins
资金
- regional government of Castilla y Leon (Spain) [UIC 071, CLU 2017-09, VA080G18]
- Ministerio de Ciencia, Innovacion y Universidades of Spain [CTQ2017-84006-C3-1-R]
- EU-FEDER [CLU 2017-09, CTQ2017-84006-C3-1-R]
- Junta de Castilla y Leon - JCyL
Biomass grown in wastewater treatment photobioreactors is a cheap raw material with high contents of carbohydrates, proteins and lipids. This work studies the production of fermentable monosaccharides from three biomasses grown in piggery wastewater (P), domestic wastewater (W) and synthetic medium (S) by applying chemical pretreatment and enzymatic hydrolysis, using a Taguchi design. ANOVA identified temperature, chemical reagent type and chemical reagent concentration as significant operational parameters. However, the biomass concentration, pretreatment time, enzyme dosage and enzymatic hydrolysis time had no remarkable effect. The bacterial content of the biomass had no relevant impact on carbohydrate and protein solubilisation but had a remarkable effect on the degradation of the released carbohydrates (57, 60 and 37% for P, W and S), while also affecting lipid solubilisation. Pretreatment with HCl 2M at 120 degrees C resulted the optimal conditions, achieving a monosaccharide recovery of 53, 59 and 80% for P, W and S biomasses, respectively.
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