期刊
BIORESOURCE TECHNOLOGY
卷 281, 期 -, 页码 343-350出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.02.059
关键词
Alkaline thermostable xylanase; Metagenome; Camel rumen; Recalcitrant compounds
资金
- Agricultural Biotechnology Research Institute of Iran (ABRII)
- Institute of Biochemistry and Biophysics (IBB)
- Iran National Science Foundation (INSF) [96009912]
The aim of this study was to isolate and characterize novel alkali-thermostable xylanase genes from the mixed genome DNA of camel rumen metagenome. In this study, a five-stage computational screening procedure was utilized to find the primary candidate enzyme with superior properties from the camel rumen metagenome. This enzyme was subjected to cloning, purification, and structural and functional characterization. It showed high thermal stability, high activity in a broad range of pH (6-11) and temperature (30-90 degrees C) and effectivity in recalcitrant lignocellulosic biomass degradation. Our results demonstrated the power of in silico analysis to discover novel alkali-thermostable xylanases, effective for the bioconversion of lignocellulosic biomass.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据