期刊
BIOPHYSICAL JOURNAL
卷 117, 期 2, 页码 377-387出版社
CELL PRESS
DOI: 10.1016/j.bpj.2019.06.008
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资金
- Intramural Section Program of the National Institute of Neurological Disorders and Stroke of the National Institutes of Health [NS002993]
- National Science Foundation [CHE-1726332]
- Kansas Bioscience Authority funds
- Fondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT) [11170223, 1171155]
- Iniciativa Cientifica Milenio of the Ministry of Economy, Development and Tourism (Chile)
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [ZIANS002993] Funding Source: NIH RePORTER
After opening, the Shakervoltage-gated potassium (K-v) channel rapidly inactivates when one of its four N-termini enters and occludes the channel pore. Although it is known that the tip of the N-terminus reaches deep into the central cavity, the conformation adopted by this domain during inactivation and the nature of its interactions with the rest of the channel remain unclear. Here, we use molecular dynamics simulations coupled with electrophysiology experiments to reveal the atomic-scale mechanisms of inactivation. We find that the first six amino acids of the N-terminus spontaneously enter the central cavity in an extended conformation, establishing hydrophobic contacts with residues lining the pore. A second portion of the N-terminus, consisting of a long 24 amino acid alpha-helix, forms numerous polar contacts with residues in the intracellular entryway of the T1 domain. Double mutant cycle analysis revealed a strong relationship between predicted interatomic distances and empirically observed thermodynamic coupling, establishing a plausible model of the transition of K-v channels to the inactivated state.
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