期刊
BIOFOULING
卷 35, 期 5, 页码 541-550出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/08927014.2019.1627337
关键词
BsrA; repressor; biofilm synthesis; Pseudomonas aeruginosa
资金
- National Natural Science Foundation of China [31370205, 41606157]
- Natural Science Foundation of Tianjin, China [16JCYBJC20900]
Biofilm formation is an important virulence factor which is controlled by complex regulatory circuits in Pseudomonas aeruginosa. In this work, a biofilm hyper-producing strain, P2-7, was selected from a collection of transposon insertion mutants in which the PA2121 gene was disrupted. PA2121 was predicted as a putative LysR-type regulator. Analyses showed that it was involved in early biofilm formation, mature biofilm development, and colony morphology. Quantitative measurements revealed that PA2121 repressed biosynthesis of extracellular polysaccharides (alginate, psl and pel). Furthermore, it was observed that PA2121 was self-regulated, highly expressed in the early phase of biofilm development, and subject to the negative regulation by a biofilm synthesis regulator SrpA that binds directly to the PA2121 gene promoter. Collectively, this study proposes that PA2121 is a novel biofilm synthesis repressor (BsrA) in P. aeruginosa.
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