期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 190, 期 1, 页码 218-231出版社
SPRINGER
DOI: 10.1007/s12010-019-03077-z
关键词
Candida rugosa lipase 1; Pichia pastoris; Cell surface displaying; Whole-cell catalyst; Docosahexaenoic acid
资金
- National Natural Science Foundation of China [NSFC31170078]
- National High Technology Research and Development Program of China [2013AA065805, 2014AA093510]
- National Natural Science Foundation of Hubei Province [2015CFA085]
- Fundamental Research Funds for HUST [2014QN119, 2014NY007]
Yeast surface display has emerged as a viable approach for self-immobilization enzyme as whole-cell catalysts. Herein, we displayed Candida rugosa lipase 1 (CRL LIP1) on the cell wall of Pichia pastoris for docosahexaenoic acid (DHA) enrichment in algae oil. After a 96-h culture, the displayed CRL LIP1 achieved the highest activity (380 +/- 2.8 U/g) for hydrolyzing olive oil under optimal pH (7.5) and temperature (45 degrees C) conditions. Additionally, we improved the thermal stability of displayed LIP1, enabling retention of 50% of its initial bioactivity following 6 h of incubation at 45 degrees C. Furthermore, the content of DHA enhanced from 40.61% in original algae oil to 50.44% in glyceride, resulting in a 1.24-fold increase in yield. The displayed CRL LIP1 exhibited an improved thermal stability and a high degree of bioactivity toward its native macromolecule substrates algae oil and olive oil, thereby expanding its potential for industrial applications in fields of food and pharmaceutical. These results suggested that surface display provides an effective strategy for simultaneous convenient expression and target protein immobilization.
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