期刊
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
卷 63, 期 8, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.00629-19
关键词
CRISPRi; mycobacteria; molecular genetics
资金
- Maurice Wilkins Centre for Molecular Biodiscovery
- Health Research Council of New Zealand
There is an urgent need for novel therapeutics to treat Mycobacterium tuberculosis infections. Genetic strategies for validating novel targets are available, yet their time-consuming nature limits their utility. Here, using MmpL3 as a model target, we report on the application of mycobacterial CRISPR interference for the rapid validation of target essentiality and compound mode of action. This strategy has the potential to rapidly accelerate tuberculosis drug discovery.
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