期刊
ANTICANCER RESEARCH
卷 39, 期 7, 页码 3719-3725出版社
INT INST ANTICANCER RESEARCH
DOI: 10.21873/anticanres.13520
关键词
IL-24; prostate cancer; anchorage dependence; stable retroviral transfection
类别
资金
- JSPS KAKENHI [JP 26430169]
Background: Hormone therapy and chemotherapy are not effective for castrate-resistant prostate cancer, thus development of novel treatment strategies is required. Gene therapy involving transient high-copy transfection of interleukin (IL)-24 with an adenoviral vector can exert antitumor activity; however, the effects of stable IL-24 transfection are not fully understood. The aim of this study was to investigate the effects of IL-24 overexpression in prostate cancer cells, in vitro. Materials and Methods: DU145 cells were transfected the IL-24 gene using a retroviral vector. Apoptosis induction was investigated by the cell death detection ELISA, and the gene expression was analyzed by real time RT-PCR. Results: IL-24 transduction suppressed the growth of prostate cancer and induced tumor cell apoptosis. In addition, up-regulation of epithelial markers and down-regulation of mesenchymal markers were noted, suggesting that tumor aggressiveness was reduced. Conclusion: Introduction of IL-24 displays antitumor activity both by induction of apoptosis and regulation of anchorage dependence.
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