期刊
BIOMATERIALS
卷 67, 期 -, 页码 323-334出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2015.07.037
关键词
Biosensor; Fluorescent probe; Caspase; Image analysis; Persistent luminescence nanoparticles
资金
- National Natural Science Foundation of China [21375060, 21135002, 21121091]
- Priority development areas of The National Research Foundation for the Doctoral Program of Higher Education of China [20130091130005]
Time-resolved fluorescence technique can reduce the short-lived background luminescence and auto-fluorescence interference from cells and tissues by exerting the delay time between pulsed excitation light and signal acquisition. Here, we prepared persistent luminescence nanoparticles (PLNPs) to design a universal time-resolved fluorescence resonance energy transfer (TR-FRET) platform for biosensing, lifetime imaging of cell apoptosis and in situ lifetime quantification of intracellular caspase-3. Three kinds of PLNPs-based nanoprobes are assembled by covalently binding dye-labeled peptides or DNA to carboxyl-functionalized PLNPs for the efficient detection of caspase-3, microRNA and protein. The peptides-functionalized nanoprobe is also employed for fluorescence lifetime imaging to monitor cell apoptosis, which shows a dependence of cellular fluorescence lifetime on caspase-3 activity and thus leads to an in situ quantification method. This work provides a proof-of-concept for PLNPs-based TR-FRET analysis and demonstrates its potential in exploring dynamical information of life process. (C) 2015 Elsevier Ltd. All rights reserved.
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