期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 58, 期 40, 页码 14167-14172出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201908105
关键词
DNAzyme; fluorescence; phenylboronate; phosphorothioate; reactive oxygen species
资金
- National Natural Science Foundation of China [21675097, 21621003, 21707079]
RNA-cleaving DNAzymes are useful tools for intracellular metal-ion sensing and gene regulation. Incorporating stimuli-responsive modifications into these DNAzymes enables their activities to be spatiotemporally and chemically controlled for more precise applications. Despite the successful development of many caged DNAzymes for light-induced activation, DNAzymes that can be intracellularly activated by chemical inputs of biological importance, such as reactive oxygen species (ROS), are still scarce. ROS like hydrogen peroxide (H2O2) and hypochlorite (HClO) are critical mediators of oxidative stress-related cell signaling and dysregulation including activation of immune system as well as progression of diseases and aging. Herein, we report ROS-activable DNAzymes by introducing phenylboronate and phosphorothioate modifications to the Zn2+-dependent 8-17 DNAzyme. These ROS-activable DNAzymes were orthogonally activated by H2O2 and HClO inside live human and mouse cells.
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