Rational Design of Specific Recognition Molecules for Simultaneously Monitoring of Endogenous Polysulfide and Hydrogen Sulfide in the Mouse Brain

A biosensor was created for the simultaneous monitoring of endogenous H2Sn and H2S in mouse brains and exploring their roles in activation of the TRPA1 channel under two types of brain disease models: ischemia and Alzheimer's disease (AD). Based on DFT calculations and electrochemical measurements, two probes, 3,4-bis((2-fluoro-5-nitrobenzoyl)oxy)-benzoic acid (MPS-1) and N-(4-(2,5-dinitrophenoxy) phenyl)-5-(1, 2-dithiolan-3-yl)pentanamide (MHS-1), were synthesized for specific recognition of H2Sn and H2S. Through co-assembly of the two probes at the mesoporous gold film with good anti-biofouling ability and electrocatalytic activity, this microsensor showed high selectivity for H2Sn and H2S against potential biological interferences. The biosensor can simultaneously determine the concentration of H2Sn from 0.2 to 50 mu m, as well as that of H2S from 0.2 to 40 mu m. The expression of TRPA1 protein positively correlated with levels of H2Sn under both ischemia and AD.