期刊
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
卷 61, 期 6, 页码 727-736出版社
AMER THORACIC SOC
DOI: 10.1165/rcmb.2019-0070OC
关键词
alveolar macrophage; TIM-3; pulmonary fibrosis; IPF
资金
- National Key Research and Development Program of China [2016YFC1300600]
- National Open Research Major Project of China [ALJ17J002]
- National Science and Technology Major Project of China [2018ZX09J18111, 2018ZX09J18108-002]
- National Natural Sciences Foundation of China [81600042, 81771684]
- Beijing Natural Science Foundation of China [7192145]
Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive lung disorder and lacks effective treatments because of unclear mechanisms. Aberrant function of alveolarmacrophages is directly linked to pulmonary fibrosis. Here, we show TIM-3 (T-cell immunoglobulin domain and mucin domain-3), a key regulator of macrophage function, aggravates pulmonary fibrosis. TIM-3 mRNA of patients with IPF was analyzed based on the Gene Expression Omnibus and Array Express databases. Lung pathology and profibrotic molecules were assessed in a bleomycin (BLM)-induced pulmonary fibrosis model using wild-type (WT) and TIM-3 transgenic (TIM-3-TG) mice. Macrophage cells, RAW264.7, were then applied to investigate the effect of macrophage TIM-3 under BLM exposure in vitro. Macrophage depletion and adoptivetransfer experiments were finally performed to examine lung morphology and profibrotic molecules. TIM-3 expression was increased both in patients with IPF and in our BLM-inducedmouse model. TIM-3-TG mice developed more serious pathological changes in lung tissue and higher expressions of TGF-beta 1 (transforming growth factor-beta 1) and IL-10 than WT mice. After BLM treatment, TGF-beta 1 and IL-10 expression was significantly decreased in RAW264.7 cells after TIM-3 knock-out, whereas itwas increased in TIM-3-TG peritoneal macrophages. The scores of pulmonary fibrosis in WT and TIM-3-TG mice were significantly reduced, and there was no difference between them after macrophage depletion. Furthermore, WT mice receiving adoptive macrophages from TIM-3-TG mice also had more serious lung fibrosis and increased expression of TGF-beta 1 and IL-10 than those receiving macrophages from WT mice. Our findings revealed that overexpressed TIM-3 in alveolar macrophages aggravated pulmonary fibrosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据