期刊
AGING-US
卷 11, 期 13, 页码 4367-4381出版社
IMPACT JOURNALS LLC
DOI: 10.18632/aging.102058
关键词
vaccination; antibody responses; B cell metabolism
资金
- National Institute on Aging, Duke Pepper Older Americans Independence Center, NIA [P30AG028716]
- NIH [HHSN2722011000186]
Antibody responses to vaccinations or infections decline upon aging. In this study we tested if metabolic changes in B cells may contribute to attenuation of responses to influenza vaccination in aged humans. Our data show that aging affects mitochondria! functions in B cells leading to increases in mitochondria! reactive oxygen species (MROS) and mitochondria! mass (MM) in some aged B cell subsets and decreases in expression levels of Sirtuin 1 (SIRT1), Forkhead box protein (FOX)O1 and carnitine palmitoyltransferase 1 (CPT-1). Seahorse analyses showed minor defects in glycolysis in the aged B cells after activation but a strong reduction in oxidative phosphorylation. The analyses of the transcriptome revealed further pronounced defects in one carbon metabolism, a pathway that is essential for amino acid and nucleotide metabolism. Overall our data support the notion that the declining ability of aged B cells to increase their metabolism following activation contributes to the weakened antibody responses of the elderly.
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