期刊
BIOMATERIALS
卷 53, 期 -, 页码 285-295出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2015.02.106
关键词
Hypochlorite; Iridium(III) complex; Two-photon probe; Mitochondria; Zebrafish
资金
- 973 program [2014CB845604, 2015CB856301]
- National Science Foundation of China [21172273, 21171177, 21471164, J1103305]
- Program for Changjiang Scholars and Innovative Research Team in University of China [IRT1298]
Endogenous hypochlorite ion (ClO-) is a highly reactive oxygen species (ROS) that is produced from hydrogen peroxide and chloride ions catalyzed by myeloperoxidase (MPO). And mitochondrion is one of the major sources of ROS including ClO-. In the present work, a two-photon phosphorescent probe for ClO- in mitochondria was developed. An iridium(III) complex bearing a diaminomaleonitrile group as ClO- reactive moiety specifically responded to ClO- over other ions and ROSs. When the probe was reacted with ClO- to form an oxidized carboxylate product, a significant enhancement in phosphorescence intensity was observed under one-photon (402 nm) and two-photon (750 nm) excitation, with a two-photon absorption cross-section of 78.1 GM at 750 nm. More importantly, ICP-MS results and cellular images co-stained with Mito-tracker Green demonstrated that this probe possessed high specificity for mitochondria. This probe was applied in the one- and two-photon imaging of ClO- in vitro and in vivo. The results suggested endotoxin lipopolysaccharide (LPS) induced ClO- mostly generated in the liver of zebrafish. (C) 2015 Elsevier Ltd. All rights reserved.
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