期刊
ACS NANO
卷 13, 期 8, 页码 8609-8617出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.9b02771
关键词
time-resolved digital immunoassay; procalcitonin; plasmonic imaging; rapid and sensitive quantification; single nanoparticles analysis
类别
资金
- NIH [1R01GM124335]
Timely diagnosis of acute diseases improves treatment outcomes and saves lives, but it requires fast and precision quantification of biomarkers. Here, we report a time-resolved digital immunoassay based on plasmonic imaging of binding of single nanoparticles to biomarkers captured on a sensor surface. The real-time and high contrast of plasmonic imaging lead to fast and precise counting of the individual biomarkers over a wide dynamic range. We demonstrated the detection principle, evaluated the performance of the method using procalcitonin (PCT) as an example, and achieved a limit of detection of similar to 2.8 pg/mL, dynamic range of 4.2-12500 pg/mL, for a total detection time of similar to 25 min.
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